Bench Screen Evaluation of Turf Pro Against Sting Nematodes
By the University of Florida
Research Plan: This research evaluated the nematode activity of Turf Pro against
Belonolaimus longicaudatus (sting nematode). Sting nematode is the most damaging
nematode to turfgrasses in the region and also is the primary nematode affecting
strawberry, potato, and corn in Florida, and also affects numerous other crops in
Bench Screen: The objective of the bench screen outlined is to evaluate the potential
of experimental materials to kill sting nematodes. 200 cm3 aliquots of soil were
measured out and placed into a 3 x 3 x 3-inch plastic pots. A solution of Turf Pro
prepared at a concentration of 170 PPM, which was compared to untreated control (water
only). Therefore, there were 2 treatments (1 experimental + untreated). There were
8 replications of each treatment. The solutions were applied as a topical drench
in 100 ml solution to each pot. This was enough to replace the existing water in
the soil with the solutions. The untreated pots received 100 ml water. The pots were
randomized on a lab bench and left for 72 hours so that the nematodes were exposed
to the treatments for three days. Next the soil from each pot was washed onto a modified
Baermann funnels for nematode extraction. This allows live nematodes to move out
of the soil, through a filter, into water so that they can be counted while dead
nematodes staid in the soil. After incubating the modified Baermann funnels for 48
hours, the live nematodes are left behind. Nematode counts from each treatment were
counted and then compared.
Table 1. Number of live sting nematodes recovered from each treatment.
Treatment Rate (PPM in soil solution) Sting nematodes
Untreated 0 62 a
Turf Pro 170 32 b
Data are means of 8 replications. Means followed by common letters are not different
(P ≤ 0.05) according to Duncan’s multiple-range test.
These results indicate that exposure for 72 hours to TurfPro at 170 PPM can disable
or kill sting nematodes. For most golf courses, ¼-inch of water is typical for irrigation,
and also is what I consider sufficient to move chemicals into the turf nematode treatment
zone (around 2 inches deep). Based on this, 1.16 gal/acre would give approximately
170 PPM in soil solution in the treatment zone. While this preliminary experiment
looks promising, I would not draw any conclusions or recommendations without a great
deal of further experimentation. A lot can happen between applications to soil in
the lab and in the field. That being said, I am hopeful that we can follow up with
further testing since these results look promising.
William T. Crow
Associate Professor of Nematology
University of Florida
The next phase of testing, field testing, has been authorized and is underway.
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