Turf Pro USA – Products

 

Natural Occurring Bacterial Species found in TurfPro Products

 

Bacillus mojavensis  - AMH 100  

Motile, gram-positive, spore-forming natural soil isolate from ancient humified peat deposit in Georgia. Enzymes: strong cellulase, also lecithinase, gelatinase, amylase, choline oxidase, sulfide oxidase and protease. VP positive. Tolerates 7.5% NaCl aerobically, but not under anaerobic conditions.   Oxidizes sulfide and is nitrate reducing.  

Ferments (API50CH): Glycerol, L-arabinose, Ribose, D-Xylose, D-Glucose, D-Fructose, D-Mannose, Rhamnose,  Inositol, Mannitol, Sorbitol, alpha-methyl-D-glucoside, Amygdaline, Arbutine, Esculin, Salicine,  Cellobiose, Maltose, Melibiose, Saccharose, Trehalose, Inuline, D-Raffinose, Amidon, Glycogene and D-turanose.. Utilizes sucrose aerobically.

Antibiotic sensitivity: Sensitive to Ampicillin, Cephalothin, Gentamicin, Tetracycline, Clindamycin, Penicillin, & Vancomycin. Resistant to Ceftriaxone, Chloramphenicol, Erythromycin & Lincomycin.  

API 50CH/20E identification: 96.7% Bacillus subtilis

16S rRNA: 99.91% B. mojavensis (99.72% B. subtilis and Brevibacterium halotolerans)

 

Bacillus subtilis - AMH 102

Motile, gram positive, spore forming natural soil isolate from ancient humified peat soil in Georgia. VP positive. Reduces nitrate. Slow ammonia oxidation. Slight growth at pH 4.5. Produces biosurfactant. Grows at 7.5% salt.

Enzymes: gelatinase, amylase, casease, catalase, cytochrome oxidase,

Ferments (API50CH): Glycerol, Ribose, D-Glucose, D-Fructose, D-Mannose, Inositol, Mannitol, alpha-Methyl-D-Glucoside, Amygdaline, Arbutine, Esculine, Salicine, Cellobiose, Maltose, Lactose, Saccharose, Trehalose, and D-Raffinose.

API 50CH/20E identification: 75.9% Bacillus subtilis with 7 against

Antibiotic sensitivity: Sensitive to Ampicillin, Cephalothin, Chloramphenicol, Gentamicin, Tetracycline, Clindamycin, Erythromycin, Penicillin, Trimethoprim/Sulfoxazole, & Vancomycin. Resistant to Ceftriaxone. Intermediate to Lincomycin.

 

New species (proposed Nitrobacillus georgiensis) - AMH 103 (a.k.a. 021.6)

Motile, gram-positive,  aerobic strain isolated from ancient humified peat soil in Georgia. Will not ferment sugars without at least 0.5% NaCl, but will not grow well above 5% NaCl. Anaerobic starch degrader.  Oxidizes ammonia. Grows extremely well at pH 4.5 with 0.5% salt present (may be its optimum pH).  Utilizes citrate, but then nitrifies ammonia formed, so pH is increased again. (Proposed name Nitrobacillus georgiensis). Grows slowly on sulfide-nitrate agar (no carbon source present). Does not utilize thiosulfate. Produces biosurfactant.

Enzymes: very strong and fast urease, cellulase, gelatinase, catalase, cytochrome oxidase, ammonia monooxygenase,

Ferments (API50CH): D-Fructose, Arbutine and Esculine

API 50CH/20E identification: 98.1% Brevibacillus brevis with 3 against

FAME = Bacillus GC Group 22** 0.526

16S rRNA: 94.36% Kurthia gibsonii, 94.22% Bacillus insolitus (B. brevis not even in top 10)

Antibiotic sensitivity: Sensitive to Ampicillin, Cephalothin, Gentamicin, Clindamycin, Penicillin, Trimethoprim/Sulfoxazole, Vancomycin and Lincomycin.  Resistant to Ceftriaxone & Erythromycin, Intermediate to Chloramphenicol & Tetracycline

 

 

Bacillus sp. - AMH 104   

Non-motile, gram-positive strain isolated from ancient humified peat soil in Georgia. Methyl Red positive. Denitrifies to nitrogen gas.  Slow ammonia oxidation. Strong growth at pH 5. Grows at pH 4.5 in presence of 0.5% salt. Strong production of biosurfactant. VP positive.  Growth at 7.5% NaCl.

Enzymes: gelatinase, amylase, casease, lecithinase, cellulase, catalase, cytochrome oxidase,

Ferments (API50CH): Glycerol, Ribose, D-Glucose, D-Fructose, D-Mannose, Inositol, Mannitol, Sorbitol, Mannitol, alpha-Methyl-D-Glucoside, Esculine, Cellobiose, Maltose, Saccharose and Trehalose.

API 50CH/20E identification: Low discrimination 43.6% B. stearothermophilus with 3 against

Antibiotic sensitivity: Sensitive to Ampicillin, Cephalothin, Chloramphenicol, Gentamicin, Clindamycin, Penicillin, Trimethoprim/Sulfoxazole & Vancomycin. Resistant to Ceftriaxone & Lincomycin. Intermediate to Tetracycline & Erythromycin.

 

Paenibacillus polymyxa - AMH 105

Motile, gram-positive, spore forming natural strain isolate from ancient humified peat soil in Georgia. VP  positive. Reduces nitrate. Grows at pH 5 even without salt. Grows at pH 4.5 with 0.5% salt. Produces biosurfactant. Grows well at 7.5% NaCl.

Enzymes: gelatinase, amylase, casease, cellulase, and catalase.

Ferments (API50CH): Glycerol, L-Arabinose, Ribose, D-Xylose, D-Glucose, D-Fructose, D-Mannose, Inositol, Mannitol, alpha-Methyl-D-glucoside, Amygdaline, Arbutine, Esuline, Salicine, Cellobiose, Maltose, Lactose, Melibiose, Saccharose, Trehalose, D-Raffinose, Amidon, Glycogene, beta-Gentiobiose, and D-Turanose.

API 50CH/20E identification: 60.3% Paenibacillus polymyxa

Antibiotic sensitivity: Sensitive to Amikacin, Ampicillin, Cephalothin, Gentamicin, Clindamycin, Erythromycin, Penicillin, Trimethoprim/Sulfoxazole, Vancomycin and Lincomycin. Resistant to Ceftriaxone and Chloramphenicol.  Intermediate to Tetracycline

 

Bacillus megaterium - AMH 106

Motile, gram-positive natural soil isolate from ancient humified peat soil in Georgia. Enzymes: gelatinase, catalase, casease, lipase, and cytochrome oxidase. Slight growth on thiosulfate. Grows on sulfide-nitrate agar and broth.

Ferments (API50CH): Glycerol, Glucose, Mannitol and Glycogene

API 50CH/20E identification: 80.4% Bacillus megaterium with 2 against

Antibiotic sensitivities: Sensitive to Ampicillin, Cephalothin, Gentamicin, Tetracycline, Clindamycin, Penicillin, Tribrissen/Sulfoxazole & Vancomycin. Resistant to Chloramphenicol, Erythromycin & Lincomycin. Intermediate to Ceftriaxone.

 

Bacillus amyloliquefaciens - AMH 107

Spore-forming, natural soil isolate from ancient humified peat deposit in Georgia. Aerobic strain with slight anaerobic ability.  Nitrate reducing. Grows at pH 4.5 in presence of 0.5% NaCl. VP positive. Slightly more resistant to copper than other strains and grows well in  7.5% NaCl solution. Strong producer of biosurfactant. Enzymes: strong amylase and gelatinase enzymes, also lecithinase and lipase.

Ferments (API50CH): Glycerol, L-Arabinose, Ribose, D-glucose, D-Fructose, D-Mannose, Inositol, Mannitol, Sorbitol, Esculine, Salicine, Cellobiose, Maltose, Trehalose, Amidon, and Glycogene.

 

API 50CH/20E identification: 98.3% Bacillus subtilis with 5 against  (only 1.1% B. amyloliquefaciens)

16S rRNA: 99.91% Bacillus amyloliquefaciens or Bacillus atrophaeus (no pigment for this one), or 99.25% B. mojavensis.

Antibiotic sensitivity: Sensitive to Ampicillin, Cephalothin, Chloramphenicol, Gentamicin, Tetracycline,  Clindamycin, Penicillin, Trimethoprim/Sulfoxazole, & Vancomycin.  Resistant to Erythromycin & Lincomycin. Intermediate to Ceftriaxone.

 

Bacillus mojavensis AMH 108 (a.k.a. 021.3)

Motile, spore forming, gram-positive, natural soil isolate from ancient humified peat soil in Georgia. Powerful anaerobic starch degrader. Tolerates 10% NaCl. VP positive. Grows at pH 4.5 in presence of 0.5% salt. Sulfide oxidation, light utilization of thiosulfate. Enzymes: Gelatinase, amylase, lipase, catalase, and cytochrome oxidase.  Nitrate reduction. Methyl Red Negative.

Ferments (API50CH): Glycerol, L-Arabinose, Ribose, D-Glucose, D-Fructose, D-Mannose, Inositol, Mannitol, Sorbitol, alpha-Methyl-D-Glucoside, Arbutine, Esculine, Cellobiose, Maltose, Melibiose, Saccharose, Trehalose, D-Raffinose, Amidon, Glycogene, beta-Gentiobiose, and D-Tagatose.

API 50CH/20E identification: 94% Bacillus subtilis with 3 against

FAME = B. subtilis 0.619 or B. amyloliquefaciens 0.598

16S rRNA: 100% Bacillus mojavensis, 99.81% Bacillus subtilis or Brevibacillus halotolerans

Antibiotic sensitivity: Sensitive to Ampicillin, Cephalothin, Chloramphenicol, Gentamicin, Clindamycin, Penicillin, Trimethoprim/Sulfoxazole, Vancomycin & Nalidixic acid. Resistant to Lincomycin. Intermediate to Ceftriaxone & Tetracycline

 

Bacillus pumilus - AMH 109

Spore-forming, motile, aerotolerant anaerobic natural soil isolate from ancient humified peat deposit in Georgia. Grows well up to 10% NaCl, both aerobically and anaerobically. Grows at pH 4.5 in presence of 0.5% salt. VP positive. Strong lipase, esterase and biosurfactant production. Other enzymes produced include nitrate reductase, nitrite reductase, sulfide oxidase, gelatinase, catalase and cytochrome oxidase.  Powerful anaerobic starch degrader. Sulfide oxidizer, either aerobically or anaerobically in presence of nitrate.  

Ferments (API50CH): Glycerol, Arabinose, Ribose, D-Xylose, Galactose, D-Glucose, D-Fructose, D-Mannose, Mannitol, N acetyl glucosamine, Amygdaline, Arbutine, Esculine, Salicine, Cellobiose, Maltose, Saccharose, Trehalose, D-Raffinose, Amidon, Glycogene, and D-Tagatose.

API 50CH/20E identification: 95.7% Bacillus licheniformis with 4 against

16S rRNA: 99.71% Bacillus pumilus, 97.67% B. atrophaeus

Antibiotic sensitivity: Sensitive to Ampicillin, Cephalothin, Gentamicin, Tetracylcine, Clindamycin Amoxicillin/Clavulanic acid, Vancomycin and Penicillin. Resistant to: Chloramphenicol, Erythromycin, Trimethoprim/Sulfa, and Lincomycin. Intermediate to Cefuroxime Axetil and Ceftriaxone.

 

Bacillus pumilus - AMH 111

Gram-positive, natural strain isolated from ancient humified peat soil in Georgia. Utilizes citrate as a sole source of carbon. Oxidizes thiosulfate and sulfide. VP positive. Grows at pH 5. Grows at pH 4.5 with 0.5% salt. Produces biosurfactant. Enzymes: gelatinase, amylase, slow lipase, slight lecithinase, catalase, cytochrome oxidase,

Ferments API50CH: Glycerol, L-Arabinose, Ribose, D-Xylose, Galactose, D-Glucose, D-Fructose, D-Mannose, Mannitol, Amygdaline, Arbutine, Esculine, Salicine, Cellobiose, Saccharose, Trehalose, Amidon, beta-Gentiobiose, and D-Tagatose

API 50CH/20E identification: 99.9% Bacillus pumilus with 2 against

Antibiotic Sensitivity: Sensitive to Ampicillin, Gentamycin, Tetracycline, Penicillin,

 

Trimethoprim/Sulfoxazole, Amoxicillin/Clavulanic Acid, Polymyxin B, Vancomycin

Resistant to Cephalothin, Ceftriaxone, Chloramphenicol, Clindamycin, Erythromycin, and Lincomycin

Bacillus pumilus - AMH 114 (a.k.a. 021.5)

Spore-forming, motile, facultatively anaerobic natural soil isolate from ancient humified peat deposit in Georgia. Slight oxidation of thiosulfate.   Strong growth at pH 4.5, as long as at least 0.5% NaCl is present. Grows well at 10% NaCl, both aerobically and anaerobically. ONPG positive. VP positive. Produces biosurfactant.

Enzymes: Strong lipase, esterase, and catalase, cellulase, slight amylase and lecithinase

Ferments (API 50CH): Ferments Esculine, D-glucose, Mannitol

API 50CH/20E identification: 95.4% Brevibacillus brevis with 1 against

FAME = B.pumilus 0.792 (other than type strain)

16S rRNA: 100% Bacillus pumilus (nothing else close)

Antibiotic Sensitivity: Sensitive to: Ampicillin, Chloramphenicol, Gentamycin, Tetracycline, Penicillin, Amoxicillin/Clavulanic acid, Vancomycin

Resistant to: Cephalothin, Ceftriaxone, Clindamycin, Erythromycin, Trimethoprim/Sulfoxazole, Lincomycin

 

Bacillus pumilus - AMH 115 (a.k.a. 021.4)

Spore-forming, motile, gram-positive rod shaped natural soil isolate from ancient humified peat deposit in Georgia.  Grows well at 10% NaCl, both aerobically and anaerobically.   Grows at  pH 4.5, as long as at least 0.5% NaCl is present. VP positive. Citrate positive. ONPG positive. Produces biosurfactant. Slight oxidation of thiosulfate and sulfide.

Enzymes: strong lipase, esterase, lecithinase, catalase, cellulase, nitrate reductase, gelatinase,

Methyl Red positive.

Ferments (API50CH): D-Glucose, D-Fructose, Mannitol, Arbutine, Esculine, Salicine, Maltose, Saccharose, and D-Tagatose. Very slight use of citrate as a sole carbon source.

API 50CH/20E identification: 98.4% Brevibacillus brevis with 2 against

FAME= B. pumilus 0.806 (other than type strain)

16S rRNA: 100% Bacillus pumilus (nothing else close)

Antibiotic sensitivity: Sensitive to Ampicillin, Cephalothin, Gentamicin, Tetracylcine, Clindamycin Amoxicillin/Clavulanic acid, Trimethoprim/Sulfa, Vancomycin and Penicillin. Resistant to: Chloramphenicol, Erythromycin, and Lincomycin. Intermediate to Cefuroxime Axetil and Ceftriaxone.

 

Bacillus mojavensis -  AMH 118 (a.k.a. 021.2)

Gram positive, motile, facultative anaerobe. Grows at  10% NaCl.  Powerful anaerobic starch degrader.  Oxidizes thiosulfate and  ammonia. Grows at pH 4.5 in presence of 0.5 NaCl. Nitrate reduction. Enzymes: strong cellulase and lipase, lecithinase, gelatinase, ammonia monooxygenase, cellulase and catalase.  VP positive. ONPG positive. Citrate positive.

Ferments (API50 CH): Glycerol, D-Arabinose, L-Arabinose, D-Glucose, D-Fructose, D-Mannose, Inositol, Mannitol, Sorbitol, alpha-Methyl-D-Glucoside, N-acetyl glucosamine, Amygdaline, Arbutine, Esculine, Salicine, Cellobiose, Maltose, Melibiose, Saccharose, Trehalose, Inuline, D-Raffinose, Amidon, Glycogene and D-Tagatose. On another test utilizes sucrose too.

API 50CH/20E identification: 64.6% Bacillus subtilis with 3 against

FAME = B. subtilis 0.678 or B. amyloliquefaciens 0.645

16S rRNA: 100% Bacillus mojavensis, 99.81% Bacillus subtilis or Brevibacillus halotolerans

Antibiotic sensitivity: Sensitive to Ampicillin, Cephalothin, Genamycin, Tetracycline, Penicillin, Trimethoprim.Sulfoxazole, Vancomycin

Resistant to: Ceftiaxone,  Chloramphenicol, Erythromycin, Lincomycin

 

Bacillus pumilus - AMH 119 (a.k.a. 021.7)

Gram positive, motile, facultatively anaerobic natural soil isolate from ancient humified peat deposit in Georgia. Oxidizes thiosulfate. Grows at pH 4.5 as long as 0.5% salt is present.  Grows at 10% NaCl. Could not reduce nitrate without presence of salt. Requires at least 0.5% salt. VP positive. ONPG positive. Citrate positive.

Enzymes: strong cellulase and lipase, gelatinase, slight amylase, catalase, cytochrome oxidase,

Ferments (API50 CH): D-Glucose, D-Fructose, D-Mannose, Mannitol, Arbutine, Esculine, and Salicine. On another test, ferments sucrose.

API 50CH/20E identification: 58% Brevibacillus laterosporus with 4 against

FAME = B. pumilus 0.903 (other than type strain)

16S rRNA: test failed

Antibiotic sensitivity: Sensitive to: Ampicillin, Cephalothin, Ceftriaxone, Chloramphenicol, Gentamycin, Tetracycline, Clindamycin, Penicillin, Trimethoprim/Sulfoxazole, Amoxicillin/Clavulanic acid, Lincomycin, Vancomycin

Resistant to: Erythromycin

 

Eupenicillium sp.

Target Pests: Eupenicillium and Penicillium were examined for their ability to grow on the spore-producing structures of Aspergillus and thereby interfere with their ability to reproduce. The Eupenicillium and Penicillium species were shown to be host specific and restricted to either A. flavus or A. niger.

 

Penicillium sp. – Three Strains

Target Pests: Eupenicillium and Penicillium were examined for their ability to grow on the spore-producing structures of Aspergillus and thereby interfere with their ability to reproduce. The Eupenicillium and Penicillium species were shown to be host specific and restricted to either A. flavus or A. niger.

 

Humicola sp.

Target Pests: No information to date.

Other Uses: Able to hydrolyze almost all forms of natural fats.

 

TurfPro USA                                    (2024AB)                      5/9/2011

 

 

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